Coding

Part:BBa_K1796014:Design

Designed by: Nannan Xie   Group: iGEM15_SCU_China   (2015-09-17)


nifV promoted from Paenibacillus sp. WLY78


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1106
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1110
    Illegal BamHI site found at 933
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1106
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1106
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves.Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But the part can be assembled by gibson assembly,that is what we did.


Source

It is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.

References